单细胞 | 批量 FeaturePlot 可视化n个基因表达量

CITE-seq中的关键膜蛋白标记及FeaturePlot应用

最新推荐文章于 2025-06-06 09:45:54 发布

原创最新推荐文章于 2025-06-06 09:45:54 发布 · 2.3k 阅读

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#数据可视化

文章讨论了CITE-seq中CD5,CD7,TNFRSF8等膜蛋白marker的重要性，并展示了如何使用FeaturePlot进行多特征分析，包括CD34,CD8A等标志物的批量绘图。

要点：CITE-seq 的膜蛋白marker的意义；批量绘制 FeaturePlot。

T: CD5, CD7, TNFRSF8(CD30)?, NCAM1(CD56)?
mono: CD33, ANPEP(CD13), FCGR1A(CD64), FUT4(CD15)?, IL3RA(CD123)
B: MME(CD10), CD38
ery: TFRC(CD71),
HSC: CD34, KIT(CD117),

1,feature plot with no axis

在这里插入图片描述

2. Code

FeaturePlot(scObj, features =c("CD3D", "CD8A", "GZMB",   "CCR7", "CCL5", 
                               "CD5","CD7", 
                               
                               "HLA-DRA",
                               "LYZ", "CD33", "ANPEP", "FCGR1A", "IL3RA",
                               
                               "CD79A","CD79B","VPREB1", "IGJ",
                               "MME","CD38",
                               "HBA1", "GATA1", "TFRC",#CD71
                               "CD34", "SOCS2", 
                               "GAPDH"), 
            cols = c("lightgrey", 'red'),
            ncol = 5 ) & NoLegend() & NoAxes() & theme(
              panel.border = element_rect(color = "black", size = 1)
            )