bowtie2-build contigs.fa fre
bowtie2 -p 32 -x fre -1 reads_1.fastq -2 reads_2.fastq -S Alignment.sam
samtools view -bS Alignment.sam > Alignment.bam
samtools sort Alignment.bam > Alignment.sort.bam
samtools index Alignment.sort.bam
java -Xmx16G -jar pilon-1.22.jar --genome contigs.fa --frags Alignment.sort.bam --fix snps,indels --output pilon_polished --vcf &> pilon.log
扫一扫
1856
被折叠的 条评论
为什么被折叠?
到【灌水乐园】发言
