ROS-ID®缺氧/氧化应激检测试剂盒

Enzo Life Sciences的ROS-ID®Hypoxia/Oxidative stress detection kit专门用于使用荧光显微镜或流式细胞术对活细胞（悬浮和贴壁）中的缺氧和氧化应激水平进行功能性检测。该试剂盒包含能够检测缺氧状态（红色）和氧化应激水平（绿色）的荧光探针。

检测缺氧的染料（红色）利用缺氧细胞中存在的硝基还原酶活性，将硝基基团转化为羟胺（NHOH）和氨基（NH2）并释放出荧光探针。

氧化应激检测试剂（绿色）是一种非荧光的、可透过细胞的总ROS检测染料，可与多种活性物质直接反应。可使用配备标准荧光素（490/525 nm）和德克萨斯红（596/670 nm）滤光片的宽场荧光显微镜、共聚焦显微镜或配备蓝色（488 nm）激光的流式细胞仪进行细胞分析。

产品特点

● 高灵敏度和特异性的荧光探针，可用于检测活细胞的缺氧和氧化应激

● 可用于分析贴壁或悬浮细胞系

● 试剂盒内包含整套试剂，包括ROS和缺氧诱导剂

实验示例

图1. 检测人HeLa和HL-60细胞中的缺氧和氧化应激水平。用缺氧诱导剂（DFO）和ROS诱导剂（pyocyanin）处理细胞。每个象限的数字反映了细胞（群体）的百分比。结果表明，缺氧和氧化应激染料具有特异性。

图2.使用不同试剂诱导HeLa细胞发生缺氧和氧化应激反应。缺氧探针在缺氧条件下被细胞硝基还原酶转化后观察到红色荧光。

图3.氧化应激（A）和缺氧（B）检测染料的吸收峰和发射峰分别为504nm/524nm和580nm/595nm。这些染料可以用488nm的氩离子激光器激发，并在流式细胞仪上的FL1通道（氧化应激染料）和FL3通道（缺氧红染料）中检测。

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产品信息

产品货号	ENZ-51042-0125/ ENZ-51042-K500
产品名称	ROS-ID® Hypoxia/Oxidative stress detection kit
别名	ROS / Nitroreductrase
规格	1*125tests/1*500tests
短期保存	-20°C
长期保存	-20°C
试剂盒组分	Hypoxia Red Detection Reagent Oxidative Stress Detection Reagent (Green) ROS Inducer (Pyocyanin) Hypoxia Inducer (DFO)
应用	Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS

  

部分产品引用文献

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