沉积物理化指标测定

文章介绍了对沉积物进行含水率、pH值、电导率和氧化还原点位等环境参数的测定方法,这些参数影响着微生物群落的结构。通过对黄海潮间带沉积物的研究,揭示了环境因素如何改变细菌群落。同时,讨论了沉积物中碳、氮、硫与微生物群落的关系及其对环境的影响。

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1、含水率
        The water content (WC) was obtained by measuring the weight loss  after the sediments were dried at 70 °C for 72 h until a constant weight  was attained. 通过测量沉积物在70°C下干燥72 h,直到达到恒定的重量后的重量损失,得到含水量(WC)[1]。
2、pH
        将沉积物风干并过2mm的筛网(网眼大小没有特定标准),称取一定量的土样并用蒸馏水浸提(水:土 = 2.5ml∶1g)[2-3],用搅拌器搅拌1min使土粒充分分散,放置30min后用pH计测定pH值。
       a pH meter (IQ Scientific Instruments)[5]
3、电导率Electric conductivity(EC)
        使用电导率仪(DDSJ-308F,INESA,China)在沉积物:水 = 1g:5ml)条件下测量沉积物的电导率(EC)[4]。
       Soil electric conductivity (EC) were determined using a 2265FS EC meter (Spectrum Technologies Inc.)[5]

4、氧化还原点位ORP

[6-7]


[1] Lee H , Heo Y M , Kwon S L ,et al.Environmental drivers affecting the bacterial community of intertidal sediments in the Yellow Sea[J].Science of The Total Environment, 2020, 755.

[2]杨雨虹,贺惠,米铁柱,等.耐盐碱水稻土壤产甲烷菌群落特征及产甲烷途径[J].环境科学, 2021, 42(7):10.
[3] Liao W , Tong D , Li Z ,et al.Characteristics of microbial community composition and its relationship with carbon, nitrogen and sulfur in sediments[J].Science of The Total Environment, 2021, 795:148848-.DOI:10.1016/j.scitotenv.2021.148848. 
[4] Li T, Chen Y, Wang Y, et al. Occurrence, source apportionment and risk assessment of perfluorinated compounds in sediments from the longest river in Asia[J]. Journal of Hazardous Materials, 2024, 467: 133608.
[5] Moderate salinity improves the availability of soil P by regulating P-cycling microbial communities in coastal wetlands
[6] Zhang W , Lei M , Li Y ,et al.Determination of vertical and horizontal assemblage drivers of bacterial community in a heavily polluted urban river[J].Water Research, 2019, 161.
[7] Sediment bacterial communities in a eutrophic lake influenced by multiple inflow-rivers
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