Be quiet

<?xml version="1.0" encoding="utf-8"?> Be quiet

Be quiet

Table of Contents

1 Be quiet

最近心情有点不太好,各方面原因。主要是25岁是个转折点。太多的转折点了。让我有些躁动与不安!

1, 来公司三年了,合同满期了。工资不如意!

2, 家里两位女生,她们每周需要进货,搬货。体力劳动啊。每周都指望着我能不加班,家里只有我一个劳力!可干我们这一行的,有很多该死的潜规则。有事没事都得加班。

3, 在公司学到的东西也越来越少。自我提升有点困难!

4, 这个年龄感觉有点老了。干这一行朋友也比较少。除了领导就是同事。圈子太窄。

5, 公司感觉很压抑!不像以前了!

我需要静下心来,找回自己。找到方向。

Date: 2013-08-02 Fri

Author: liweilijie

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转载于:https://www.cnblogs.com/liweilijie/p/3232964.html

seqkit seq "$file" -s --id ">$seq_id" -w 100 > output.fasta Error: unknown flag: --id Usage: seqkit seq [flags] Flags: -k, --color colorize sequences - to be piped into "less -R" -p, --complement complement sequence, flag '-v' is recommended to switch on --dna2rna DNA to RNA -G, --gap-letters string gap letters to be removed with -g/--remove-gaps (default "- \t.") -h, --help help for seq -l, --lower-case print sequences in lower case -M, --max-len int only print sequences shorter than or equal to the maximum length (-1 for no limit) (default -1) -R, --max-qual float only print sequences with average quality less than this limit (-1 for no limit) (default -1) -m, --min-len int only print sequences longer than or equal to the minimum length (-1 for no limit) (default -1) -Q, --min-qual float only print sequences with average quality greater or equal than this limit (-1 for no limit) (default -1) -n, --name only print names/sequence headers -i, --only-id print IDs instead of full headers -q, --qual only print qualities -b, --qual-ascii-base int ASCII BASE, 33 for Phred+33 (default 33) -g, --remove-gaps remove gaps letters set by -G/--gap-letters, e.g., spaces, tabs, and dashes (gaps "-" in aligned sequences) -r, --reverse reverse sequence --rna2dna RNA to DNA -s, --seq only print sequences -u, --upper-case print sequences in upper case -v, --validate-seq validate bases according to the alphabet Global Flags: --alphabet-guess-seq-length int length of sequence prefix of the first FASTA record based on which seqkit guesses the sequence type (0 for whole seq) (default 10000) --compress-level int compression level for gzip, zstd, xz and bzip2. type "seqkit -h" for the range and default value for each format (default -1) --id-ncbi FASTA head is NCBI-style, e.g. >gi|110645304|ref|NC_002516.2| Pseud... --id-regexp string regular expression for parsing ID (default "^(\\S+)\\s?") -X, --infile-list string file of input files list (one file per line), if given, they are appended to files from cli arguments -w, --line-width int line width when outputting FASTA format (0 for no wrap) (default 60) -o, --out-file string out file ("-" for stdout, suffix .gz for gzipped out) (default "-") --quiet be quiet and do not show extra information -t, --seq-type string sequence type (dna|rna|protein|unlimit|auto) (for auto, it automatically detect by the first sequence) (default "auto") -j, --threads int number of CPUs. can also set with environment variable SEQKIT_THREADS) (default 4)什么意思
08-07
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