2024.5.15文献分享【ChIP-R: Assembling reproducible sets of ChIP-seq and ATAC-seq peaks from multiple...】

【Title】ChIP-R: Assembling reproducible sets of ChIP-seq and ATAC-seq peaks from multiple replicates

【Publication Book】Genomics

【Publication Time】2021 April

【Abstract】

Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is the primary protocol for detecting genome-wide DNA-protein interactions, and therefore a key tool for understanding transcriptional regulation. A number of factors, including low specificity of antibody and cellular heterogeneity of sample, may cause “peak” callers to output noise and experimental artefacts. Statistically combining multiple experimental replicates from the same condition could significantly enhance our ability to distinguish actual transcription factor binding events, even when peak caller accuracy and consistency of detection are compromised.

We