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AdaWong_Corner
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stranded 和 non-stranded RNA-seq
stranded 和 non-stranded RNA-seq 的表达谱分析和基因overlap研究的比较原文链接:Comparison of stranded and non-stranded RNA-seq transcriptome profiling and investigation of gene overlap背景non-stranded RNA-seq的建库存在的问题:不能确定每个转翻译 2017-12-13 17:05:32 · 2594 阅读 · 0 评论 -
Per tile sequence quality
Per tile sequence quality —Position specific failures of flowcells介绍当Per tile sequence quality显示fail或者warning,表明测序的lane或某个run中出现出现了部分故障,从而影响一些特定的区域和循环,进而使测序数据的质量下降。另外,如果read的3’端的质量是好的,就意味着存在瞬时质量损失(Tran翻译 2017-12-15 13:17:33 · 6252 阅读 · 2 评论