H3K27ac Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag

H3K27ac(组蛋白H3赖氨酸27乙酰化)抗体符合EpiCypher的批次特异性SNAP-Certified™标准，在CUT&RUN和CUT&Tag应用中具有特异性和高效的靶标富集。这需要与相关组蛋白 PTMs （使用加标对照的 SNAP-CUTANA™ K-AcylStat Panel 测定，EpiCypher RD193002）的交叉反应性 <20%（图 1 和 图5）。在不同的细胞起始条件下，一致的基因组富集结果证实了高靶标效率：CUT&RUN中500k和50k的细胞量(图2-3)，CUT&Tag中100k和10k的细胞量(图6-7)。即使在细胞数量减少的情况下，高效抗体也显示出相似的峰结构(图3和7)和高度保守的全基因组信号(图2和6)。H3K27ac与基因激活相关，并在活性增强子和启动子中富集[1]。

产品详情

产品名称:H3K27ac Antibody, SNAP-Certified™ for CUT&RUN and CUT&Tag/欣博盛生物

宿主来源:Rabbit

实验应用:CUT&RUN, CUT&Tag

免疫原:A synthetic peptide corresponding to histone H3 acetylated at lysine 27

克隆性:Monoclonal[2114-3E4]

保存温度:自收到之日起，4℃下可稳定储存1年。

验证数据

—— CUT&RUN

Figure 1: Average SNAP specificity analysis from two CUT&RUN experiments

CUT&RUN was performed as described in Figure 4. CUT&RUN sequencing reads were aligned to the unique DNA barcodes corresponding to each nucleosome in the K-AcylStat panel (x-axis). Data are expressed as a percent relative to on-target recovery (H3K27ac set to 100%). The antibody showed recovery of H3K27ac spike-in nucleosomes as well as H3K27ac nucleosomes that contain a proximal phosphorylation at S28 at both 500k and 50k cells. The antibody cross-reacts with extended acyl states (butyrylation and crotonylation) at H3K27, but these are typically low abundance in cells [2].

Figure 2: CUT&RUN genome-wide enrichment

CUT&RUN was performed as described in