rsem比对_科学网—FPKM, RPKM, RPM以及TPM的关系之见解 - 江纯阶的博文

FPKM，RPKM，RPM以及TPM的关系之见解

RPKM: Reads Per Kilobase of exon model per Million mapped reads (每千个碱基的转录每百万映射读取的reads)

FPKM: Fragments Per Kilobase of exon model per Million mapped fragments(每千个碱基的转录每百万映射读取的fragments)

RPM/CPM: Reads/Counts of exon model per Million mapped reads (每百万映射读取的reads)

TPM：Transcripts Per Kilobase of exon model per Million mapped reads (每千个碱基的转录每百万映射读取的Transcripts)

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RPKM (Reads Per Kilobase Million)

FPKM (Fragments Per Kilobase Million)

TPM(Transcripts Per Kilobase Million)

RPM (Reads per million)

CPM (Counts per million)

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FPKM (推荐软件，cufflinks/Stringtie)和RPKM (推荐软件，Range/Deseq)的计算方法基本一致，公式如下(外显子的表达)：

RPKM= total exon reads/ (mapped reads (Millions) * exon length(KB))

你可以用这个公式计算基因，外显子，转录本的