研究发现AmCBF1、2和3蛋白分别属于DREB亚家族A-1子组，AmCBF2在正常条件下高表达于茎部，对冷、旱、高盐、重金属及ABA有强烈反应。相比之下，AmCBF1和3表达较低，对刺激反应不明显，表明AmCBF家族在应对环境胁迫中各有分工，特别是AmCBF2可能参与冷、旱和重金属应激信号通路。
摘要：
Three CBF/DREB1 (C-repeat binding factor/dehydration responsive element-binding factor 1) homologues were isolated from mangrove Avicennia marina and designated AmCBF1, 2 and 3. Multiple sequence analysis showed that the three deduced proteins all contain an AP2 DNA-binding domain and two CBF signature sequences. According to the phylogenetic analysis, these proteins belong to the A-1 subgroup of the DREB subfamily. Expression analyses based on quantitative real-time PCR revealed that the AmCBF2 displayed relatively high expression under normal conditions, with the highest level in stems, while both AmCBF1 and 3 were weakly expressed without stress. The three genes also showed different responses to various environmental stimuli. The AmCBF2 was inducible by cold, drought, high salinity, heavy metals, as well as abscisic acid (ABA), and exhibited much stronger induction by cold, drought, Pb2+ or Zn2+ than by NaCl, ABA or Cd2+. In contrast, both AmCBF1 and AmCBF3 displayed insignificant changes under these stimuli. These results indicate that the three AmCBF genes play different roles in A. marina and the AmCBF2 might be involved in the signaling pathway of cold, drought and heavy metal stress response. (C) 2013 Elsevier B.V. All rights reserved.
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