Culture of Streptococcus

Transcriptomic Responses to Coaggregation between Streptococcus gordonii and Streptococcus oralis

DOI: https://doi.org/10.1128/AEM.01558-21

Routine culture of bacteria

S. gordonii DL1 (Challis; ATCC 35105) and S. oralis 34 (formerly S. sanguis 34)  were routinely cultured :

THYE medium	Todd Hewitt Broth (30 g · liter−1) yeast extract (5 g · liter−1)
Solidified THYE medium	Todd Hewitt Broth (30 g · liter−1;) yeast extract (5 g · liter−1)  Bacto agar (15 g · liter−1)
BHYG medium

Sterilize： All media were sterilized by autoclaving at 121°C for 15 min before use

Long-term storage：stocks of bacteria were maintained at −80°C in THYE medium supplemented with 50% glycerol.

The purity of cultures was checked frequently by phase-contrast microscopy and by plating aliquots on agar plates