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RSS订阅原创 Innovative Approaches to Building and Validating Alzheimer‘s Disease Models
Alzheimer's disease (AD) is a neurodegenerative disorder of unknown etiology, characterized primarily by neuronal loss, β-amyloid plaque deposition, and tau protein hyperphosphorylation.
2025-08-19 11:45:06
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原创 3D Tumor Spheroid Culture Solutions
3D cell culture better mimics the in vivo environment compared to traditional 2D methods by enabling cell-cell interactions along all axes and nutrient gradient formation.
2025-08-18 23:00:00
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原创 Multiplex Fluorescence IHC Technology: Unveiling the Mechanisms of Tumor Immune Evasion
The study found that p53-deficient tumor stem cells secrete IL-34, which drives the formation of the F5-CAFs subpopulation of TAMs through the CD36 axis, promoting immune escape.
2025-08-15 10:58:36
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原创 Unlocking the Secrets of Animal Brain Organoids: A Comprehensive Culturing Guide
This technical guide provides a detailed description of the standardized processes for the preparation, passaging, and cryopreservation of animal brain organoids.
2025-08-14 11:34:28
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原创 New Arrivial: High Sensitivity P-Tau217 Recombinant Rabbit Antibody!
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by memory decline and cognitive impairment, with more than 50 million patients worldwide and nearly 10 million patients in China.
2025-08-12 15:28:42
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原创 Chromatin Immunoprecipitation (ChIP) Handbook
ChIP (Chromatin Immunoprecipitation) is an experimental technique used to study protein-DNA interactions, enabling the detection of binding between histones, transcription factors, and specific regions of the genome.
2025-08-11 15:08:36
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原创 Decoding IBD: The Immunoregulatory Mechanism of TL1A/TNFSF15 and DR3 Signaling Pathways
Abstract TL1A (TNFSF15), a TNF family cytokine, regulates immune responses by binding death receptor 3 (DR3), influencing inflammation, apoptosis, and cytokine production.
2025-08-08 14:33:16
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原创 Cell-cultured meat: The new favorite on the future dining table
Cultured meat is edible animal muscle tissue produced through cell culture and tissue engineering techniques. The concept of this technology was first proposed in the 1930s.
2025-08-08 14:16:42
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原创 TAU biomarkers: precision diagnosis in Alzheimer‘s disease
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by memory loss and cognitive decline. Its core pathological features are β-amyloid plaques and tau protein neurofibrillary tangles.
2025-08-07 15:30:10
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原创 The Rising Star in Tumor Targets - Claudin18.2 Antibody Now Available
CLDN18.2 is a potential therapeutic target for various tumors, including gastric cancer, and plays a key role in maintaining cell junctions and tumor metastasis. Its expression is regulated by the PKC/AP1 pathway and is significantly upregulated in metasta
2025-08-06 14:59:09
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原创 Precise Predictor of Pregnancy-Related Risks
Preeclampsia is a serious pregnancy complication affecting 3%-7% of pregnant women worldwide, and it can lead to preterm birth or even maternal and neonatal death.
2025-08-05 23:00:00
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原创 Antibodies Against Retinol-Binding Protein 4 (RBP4): Uncovering Their Diagnostic Potential in Diseas
Retinol-binding protein 4 (RBP4) is a member of the lipid transport protein family, responsible for the specific transport of vitamin A in the blood. It is primarily expressed in adipocytes and hepatocytes.
2025-08-05 14:44:39
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原创 Monoclonal Antibodies Against Neurofilament Light Chain (NfL): Facilitating the Diagnosis of Neurolo
Neurofilament light chain (NFL), as a key biomarker of neuronal injury, plays an important role in the diagnosis and treatment of neurological diseases.
2025-08-04 16:36:29
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原创 Indispensable AAV Antibodies in Gene Therapy
Adeno-associated virus (AAV) is widely used as a viral vector in the field of gene therapy due to its low pathogenicity, low immunogenicity, and high-efficiency gene delivery capability.
2025-08-01 11:55:38
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原创 Antibody/Protein Labeling Dyes Selection Guide and FAQs
In biomedical research, antibody/protein labeling technology is a key tool for accurately tracking target molecules, and this article lists various methods for readers to study.
2025-07-30 10:50:58
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原创 Albison panoramic multi-labeled tissue microenvironment staining analysis
This experimental service uses TSA tyramide signal amplification technology to perform 7-10-color multiplex fluorescent labeling on thyroid cancer paraffin sections, constructs panoramic virtual sections via full-spectrum high-magnification imaging.
2025-07-30 10:29:40
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原创 Abics Multicolor Analysis Service - HALO Pathology Image Analysis
Pathological examination, the "gold standard" for diagnosis, has issues like subjectivity, inefficiency and low reproducibility, prompting scientists to seek new technologies such as digital pathology and AI.
2025-07-30 10:00:21
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原创 DC cell culture strategy
Dendritic cells (DCs) were discovered in 1973 by Steinman and Cohn as the most potent antigen-presenting cells, with a single DC capable of activating 100-3,000 T cells. DCs process tumor antigens and present them to T cells.
2025-07-29 08:46:49
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原创 TR-FRET（时间分辨荧光能量共振转移）在药物研发中的热门应用简介
TR-FRET技术结合荧光共振能量转移和时间分辨荧光，可高效检测1-10nm范围内的分子相互作用。该技术已成功应用于三大领域：1）筛选细胞因子/受体阻断剂，在自身免疫疾病和肿瘤治疗中展现潜力；2）评估PROTAC分子介导的蛋白降解，为"不可成药"靶点提供新策略；3）检测KRAS突变体与cRAF的相互作用，助力靶向抗癌药物开发（如MRTX1133）。这种均相检测方法具有操作简便、信噪比高（可达7倍）等优势，为药物筛选提供了高效工具。
2025-07-23 16:59:37
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原创 揭秘速递 -- 玻璃凝胶磷酸化蛋白凝胶
蛋白质磷酸化是一种关键的翻译后修饰，由蛋白激酶催化在丝氨酸、苏氨酸和酪氨酸残基上添加磷酸基团。该过程在细胞信号传导、周期调控和疾病发生中起重要作用。常用检测方法包括Western Blot、质谱和ELISA。新型Phos-isoSDS-PAGE技术通过金属复合物特异性结合磷酸基团，无需抗体即可分离磷酸化蛋白，适用于Western Blot和质谱分析。Abison推出的玻璃凝胶磷酸化蛋白预制胶简化了实验流程，但需注意样品处理和电泳条件优化。该技术为研究蛋白质磷酸化提供了新工具。
2025-07-21 17:01:58
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原创 类器官、球体以及细胞增殖、活力和毒性检测的综合策略
本文系统介绍了六种细胞活力检测方法：1.细胞代谢检测法（CCK-8/MTT等）比较CCK-8法操作简便、灵敏度高；2.DNA合成检测法（EdU法）较BrdU法更易扩散；3.ATP检测法（2D/3D）通过荧光素酶反应定量活细胞；4.荧光染料检测法（活/死双染）用于细胞示踪与状态分析；5.膜通透性检测法（台盼蓝/中性红）基于膜完整性判断细胞活性；6.LDH检测法通过酶活性评估细胞损伤。文章详细对比了各方法的原理、操作特点及应用场景，为细胞增殖、毒性和活力研究提供了全面的检测方案选择指南。
2025-07-16 16:59:08
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原创 从零开始的流式细胞术
流式细胞术是一种高效精准的细胞分析技术，广泛应用于免疫学、肿瘤学等领域。其核心原理包括液流系统、光信号检测（散射光和荧光信号）和电子系统处理。该技术能对细胞进行多参数分析，在免疫细胞分型、肿瘤研究、细胞周期检测等方面发挥重要作用，尤其为临床血液病诊断和治疗提供了关键支持。随着技术进步，流式细胞术在生物医学研究和临床应用中的价值将持续提升。
2025-07-15 15:52:58
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原创 【酶解法】小鼠脾脏单细胞悬液的制备指南
本文详细介绍了小鼠脾脏单细胞悬液的制备方法，重点适用于流式细胞术分析。实验采用酶解法（脾脏组织解离试剂盒）对1-2mm³脾脏组织进行37℃消化，经70μm滤网过滤、红细胞裂解液处理及离心洗涤后，获得高活性单细胞悬液。关键注意事项包括控制消化时间（约30分钟）、红细胞裂解时间（3-5分钟）及充分洗涤以减少碎片。典型产量为10⁸细胞/脾脏，CD45+、CD3+等免疫细胞标志物染色效果良好。文末提供了相关试剂耗材推荐及常见问题解决方案（如细胞产量低、活性差等）。为免疫学研究提供了可靠的技术支持。
2025-07-11 16:05:26
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原创 ABSin Multicolor Kits: Revolutionizing Cervical Cancer Research
We deciphered the tripartite crosstalk among LAMP3+ dendritic cells, Tregs, and Tex populations in cervical cancer, demonstrating their collective remodeling of immunosuppressive microenvironments via IDO1-driven tryptophan catabolism. Spatial mu
2025-07-08 17:08:03
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原创 Molecular Interaction Studies: An In-Depth Exploration
Molecular interaction studies are crucial for biopharmaceutical R&D, with technologies like SPR, BLI, and ITC serving as industry standards. SPR provides real-time label-free kinetic analysis, ideal for antibody-antigen interactions.
2025-07-04 18:02:07
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原创 The Growth Hormone Triad: GH, IGF-1, and IGFBP-3
The GH-IGF axis, comprising growth hormone (GH), insulin-like growth factor-1 (IGF-1), and insulin-like growth factor-binding protein 3 (IGFBP-3), plays a crucial role in regulating human growth, metabolism, and tissue repair. GH, secreted by the pituitary
2025-05-10 21:45:28
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原创 The PD-L1 IHC antibody has been officially launched on the market!
Introduction to PD-1/PD-L1The mechanism of action of PD-1/PD-L1 inhibitors involves blocking the interaction between PD-L1 expressed on tumor cells and PD-1 receptors on T cells. Tumor cells exhibit surface expression of PD-L1, which binds to PD-1 on T ce
2025-05-08 17:23:55
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原创 AntBio: 2025 AACR Meeting - Charting New Oncology Frontiers Together
The global benchmark for cancer research and therapeutics—the 2025 American Association for Cancer Research (AACR) Annual Meeting—will commence in the U.S. on April 25th!
2025-04-27 20:23:25
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原创 Unveiling IL-31: Why Has It Become a Hotspot in Canine Atopic Dermatitis Research
Atopic Dermatitis (AD) is a common and complex chronic inflammatory skin disease, particularly prevalent in canines. This dermatological condition not only significantly impacts the quality of life of affected dogs but also poses considerable challenges fo
2025-04-16 17:44:57
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原创 Starter Modified Antibodies: Empowering PTM Research with Precision and Versatility, Making Post-Tra
ost-translational modifications (PTMs) significantly influence the structure, function, stability, and localization of proteins. PTMs have long been a hot research topic in the National Natural Science Foundation of China (NSFC). Since 2021, the NSFC has l
2025-04-10 18:30:35
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原创 (pan)-Specific Antibodies and Custom Services: Facilitating Research on Post-Translational Modificat
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2025-04-09 17:49:15
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原创 New Arrivial: High Sensitivity P-Tau217 Recombinant Rabbit Antibody!
Alzheimer's Disease (AD) is a degenerative disease of the central nervous system with the characteristics of insidious onset, irreversible, progressive and long course of disease. The clinical manifestations are progressive memory impairment, cognitive dys
2025-04-07 17:35:04
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原创 IHC Cancer Biomarker | MMR Protein Immunohistochemistry: Key Biomarker For Predicting Tumor Immunoth
What is MMR?Mismatch repair (MMR) refers to the gene mismatch repair function, which repairs errors during DNA replication. MMR can be divided into deficient MisMatch Repair (dMMR) and proficient MisMatch Repair (pMMR). When the expression of MMR proteins
2025-04-06 13:37:45
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原创 The golden partner of CAR-T therapy! High-quality target protein, cost-effective beyond imagination!
Chimeric antigen receptor T-cell (CAR-T) therapy has emerged as a revolutionary approach in cancer treatment, offering new hope for patients with refractory malignancies. At the heart of this groundbreaking therapy lies the crucial role of target proteins
2025-04-06 13:36:59
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原创 Unveiling the Mystery of Helicobacter pylori: Detection and Significance
Helicobacter pylori (H. pylori), previously known as Campylobacter pylori, is a gram - negative, microaerophilic, spiral - shaped bacterium that makes its home in the human stomach. This unique helical shape isn't just a curious feature; it's an evolutiona
2025-04-01 22:13:43
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原创 Unveiling the Power of Mouse PD-1 Fc Chimera Protein
In the intricate world of immunology, there are molecules that hold the key to unlocking a deeper understanding of our body's defense mechanisms. One such remarkable molecule is the Mouse PD-1 Fc Chimera Protein, which has been a subject of intense researc
2025-03-31 17:41:01
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原创 The PD-L1 IHC antibody has been officially launched on the market!
The mechanism of action of PD-1/PD-L1 inhibitors involves blocking the interaction between PD-L1 expressed on tumor cells and PD-1 receptors on T cells. Tumor cells exhibit surface expression of PD-L1, which binds to PD-1 on T cells, delivering inhibitory
2025-03-31 17:20:01
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原创 Impact Factor: 27.4! START Secondary Antibodies Facilitate High-Efficiency RNA Delivery Research!
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2025-03-27 15:40:35
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原创 The Vital Role of IgM and the Power of OneStep ELISA Technology
In the complex and fascinating world of immunology, one antibody stands out as a critical player in our body’s defense system: IgM. This remarkable molecule is the first line of defense in the humoral immune response, providing essential protection against
2025-03-25 19:32:42
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原创 The Intriguing World of Antibodies and ELISA Technology
In the intricate dance of our immune system, antibodies play a crucial role. These tiny proteins are like the body’s security guards, constantly on the lookout for foreign invaders. But did you know that antibodies come in different types, each with its ow
2025-03-25 19:29:31
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