BioXCell：RecombiMAb anti-mouse Ly6G

BioXCell--RecombiMAb anti-mouse Ly6G

产品描述：

1A8-CP129单克隆抗体是原始1A8单克隆抗体的重组嵌合型抗体。可变结构域序列与原始1A8相同，但是恒定区序列已经从大鼠IgG2a变为小鼠IgG2a。1A8-CP129单克隆抗体像原始克隆号的大鼠IgG2a抗体一样，不包含Fc突变。

1A8-CP129单克隆抗体与小鼠Ly6G反应。Ly6G分子量为21-25kDa，是GPI锚定的细胞表面蛋白Ly-6超家族的成员，在细胞信号传导和细胞粘附中发挥作用。Ly6G在发育过程中由骨髓谱系中的细胞（包括单核细胞、巨噬细胞、粒细胞和嗜中性粒细胞）差异表达。单核细胞通常在发育过程中瞬时表达Ly6G，而成熟的粒细胞和外周嗜中性粒细胞持续表达，使Ly6G成为这些细胞群体的表面标志物。与BioXcell RB6-8C5单克隆抗体不同，1A8-CP129单克隆抗体与小鼠Ly6G特异性反应，而与Ly6C没有交叉反应性的报道。

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产品详情：

产品名称	RecombiMAb anti-mouse Ly6G / 欣博盛生物
产品货号	CP129
产品规格	1/5/25/50/100mg
反应种属	Mouse
克隆号	1A8-CP129
同种型	Mouse IgG2a(switched from rat IgG2a)
免疫原	EL4J cells transfected with Ly6G
实验应用	in vivo neutrophil depletion* in vivo MDSC depletion* Immunofluorescence* Immunohistochemistry (paraffin)* Immunohistochemistry (frozen)* Flow cytometry* *Reported for the original rat IgG2a 1A8 antibody
产品形式	PBS, pH 7.0,Contains no stabilizers or preservatives
纯度	>95%, Determined by SDS-PAGE
聚合	<5%, Determined by SEC
无菌处理	0.2 µm filtration
纯化方式	Protein G
分子量	150 kDa
小鼠病原检测	Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative
保存条件	抗体原液保存在4°C，不能冷冻保存。
推荐同型对照	InVivoPlus mouse IgG2a isotype control, unknown specificity（货号BP0085）
推荐抗体稀释液	InVivoPure pH 7.0 Dilution Buffer（货号IP0070）

该产品自上市已被多篇SCI文献引用，品质有保证，以下是部分已发表的文献引用：

应用	文章
体内中性粒细胞耗竭 (in vivo neutrophil depletion)	1. Davis, R. W. t., et al. (2018). 'Luminol Chemiluminescence Reports Photodynamic  Therapy-Generated Neutrophil Activity In Vivo and Serves as a Biomarker of Therapeutic  Efficacy' Photochem Photobiol . 2. Moynihan, K. D., et al. (2016). 'Eradication of large established tumors in mice by  combination immunotherapy that engages innate and adaptive immune responses' Nat Med.  doi : 10.1038/nm.4200. 3. Conde, P., et al. (2015). 'DC-SIGN(+) Macrophages Control the Induction of Transplantation  Tolerance' Immunity 42(6): 1143-1158. 4. Griseri, T., et al. (2015). 'Granulocyte Macrophage Colony-Stimulating Factor-Activated  Eosinophils Promote Interleukin-23 Driven Chronic Colitis' Immunity 43(1): 187-199. 5. Yamada, D. H., et al. (2015). 'Suppression of Fcgamma-receptor-mediated antibody  effector function during persistent viral infection' Immunity 42(2): 379-390.
体内中性粒细胞耗竭、流式细胞术、 免疫组化石蜡切片(in vivo neutrophil  depletion, Flow Cytometry,  Immunohistochemistry (paraffin))	Coffelt, S. B., et al. (2015). 'IL-17-producing gammadelta T cells and neutrophils conspire  to promote breast cancer metastasis' Nature 522(7556): 345-348.
体内中性粒细胞耗竭、流式细胞术、 免疫组化石蜡切片、免疫组化冰冻切片 (in vivo neutrophil depletion, Flow  Cytometry, Immunohistochemistry  (paraffin), Immunohistochemistry (frozen)	Finisguerra, V., et al. (2015). 'MET is required for the recruitment of anti-tumoural  neutrophils' Nature 522(7556): 349-353.
体内中性粒细胞耗竭、流式细胞术 (in vivo neutrophil depletion,  Flow Cytometry)	1.Moser, E. K., et al. (2014). 'Late engagement of CD86 after influenza virus  clearance promotes recovery in a FoxP3+ regulatory T cell dependent manner'  PLoS Pathog 10(8): e1004315. 2. Chen, K. W., et al. (2014). 'The neutrophil NLRC4 inflammasome selectively  promotes IL-1beta maturation without pyroptosis during acute Salmonella  challenge' Cell Rep 8(2): 570-582. 3. Garraud, K., et al. (2012). 'Differential role of the interleukin-17 axis and  neutrophils in resolution of inhalational anthrax' Infect Immun 80(1): 131-142.
体内骨髓来源抑制细胞耗竭 (in vivo MDSC depletion)	Deng, L., et al. (2014). 'Irradiation and anti-PD-L1 treatment synergistically  promote antitumor immunity in mice' J Clin Invest 124(2): 687-695.
体内中性粒细胞耗竭、 免疫荧光(in vivo neutrophil  depletion, Immunofluorescence)	Edelson, B. T., et al. (2011). 'CD8alpha(+) dendritic cells are an obligate  cellular entry point for productive infection by Listeria monocytogenes'  Immunity 35(2): 236-248.