signature=50bdf48687eb99a039b13996789f388e,A full-length cDNA resource for the pea aphid

最新推荐文章于 2021-05-30 16:42:19 发布

转载最新推荐文章于 2021-05-30 16:42:19 发布 · 321 阅读

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本文报道了从豌豆蚜Acyrthosiphon pisum中构建的50599个全长cDNA克隆集合，产生了97828个高质量的表达序列标签，代表约9000个基因。这些序列被整合到AphidBase中，显著改善了基因预测和手动注释。分析表明，全长cDNA可以进一步提高基因模型的准确性，甚至能发现官方基因集中未包含的新基因。这个资源可用于多种功能研究，为豌豆蚜的功能基因组学提供了宝贵的社区资源。

摘要：

Large collections of full-length cDNAs are important resources for genome annotation and functional genomics. We report the creation of a collection of 50 599 full-length cDNA clones from the pea aphid, Acyrthosiphon pisum. Sequencing from 5' and 3' ends of the clones generated 97 828 high-quality expressed sequence tags, representing approximately 9000 genes. These sequences were imported to AphidBase and are shown to play crucial roles in both automatic gene prediction and manual annotation. Our detailed analyses demonstrated that the full-length cDNAs can further improve gene models and can even identify novel genes that are not included in the current version of the official gene set. This full-length cDNA collection can be utilized for a wide variety of functional studies, serving as a community resource for the study of the functional genomics of the pea aphid.

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