signature=844143986bd8ebdb6d2c781db9543683,The Development of Macrophage-Mediated Cell Therapy to Im...-优快云博客

本文探讨了急性损伤后骨骼肌再生的复杂过程，特别强调了巨噬细胞（MPs）在这一过程中的关键角色。研究发现，M1型巨噬细胞通过移植可以改善肌肉功能恢复，而未极化的M0型巨噬细胞则可能损害肌肉功能。此外，早期使用M1型巨噬细胞能加速肌纤维修复，减少纤维化并提高IGF-I表达，从而促进整体肌肉再生。

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摘要：

Skeletal muscle regeneration following acute injury is a multi-step process involving complex changes in tissue microenvironment. Macrophages (MPs) are one of the key cell types involved in orchestration and modulation of the repair process. Multiple studies highlight the essential role of MPs in the control of the myogenic program and inflammatory response during skeletal muscle regeneration. A variety of MP phenotypes have been identified and characterized in vitro as well as in vivo. As such, MPs hold great promise for cell-based therapies in the field of regenerative medicine. In this study we used bone-marrow derived in vitro LPS/IFN-y-induced M1 MPs to enhance functional muscle recovery after tourniquet-induced ischemia/reperfusion injury (TK-I/R). We detected a 15% improvement in specific tension and force normalized to mass after M1 (LPS/IFN-γ) MP transplantation 24 hours post-reperfusion. Interestingly, we found that M0 bone marrow-derived unpolarized MPs significantly impaired muscle function highlighting the complexity of temporally coordinated skeletal muscle regenerative program. Furthermore, we show that delivery of M1 (LPS/IFN-γ) MPs early in regeneration accelerates myofiber repair, decreases fibrotic tissue deposition and increases whole muscle IGF-I expression.

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