signature=7402d3a403af3333b764e77acb252fe6,Ultrasensitive Detection of Hepatitis C Virus DNA Subtype...

Chem. Res. Chinese Universities, 2020, 36(2), 307一 312 doi: 10.1007/s40242-020-9111-8

Article

Ultrasensitive Detection of Hepatitis C Virus DNA

Subtypes Based on Cucurbituril and Graphene

Oxide Nano-composite

JIANG Ping12, LI Yifei2, JU Ting1'2, CHENG Wenbo3'4, XU Jianhua5 and HAN Kun1'2'3*

1. University o f Science and Technology o f China, Hefei 230027, P. R. China;

2. CAS Key Lab o f Bio-medical Diagnostics, Suzhou Institute o f Biomedical Engineering and Technology,

Chinese Academy o f Sciences, Suzhou 215163, P. R. China;

3. University o f Chinese Academy o f Sciences, Beijing 100049, P. R China;

4. Institute o f Optics and Electronics, Chengdu 610209, P. R. China;

5. Laboratory o f Oncology Science and Molecular Biology,

Shunde Hospital o f Guangzhou University o f Chinese Medicine, Foshan 528333, P. R. China

Abstract Infectious of hepatitis C viruses(HCVs) lead to hepatic fibrosis, cirrhosis even hepatoma. Developing rapid

and sensitive diagnostic m e t h o d for H C V is of great importance. Based on the host-and-guest interaction between

cucurbit[7]uril(CB[7]) and methylene blue(MB), a CB[7]-graphene nano-composite(CB[7]-N 3- G O ) is raised for the

electrochemical detection of H C V D N A . Tlie method is able to linearly detect the H C V nucleic acid in the range of

0.2— 10 nmol/L with detection limit as low as 160.4 pmol/L. T h e proposed detection strategy is able to discriminate

the lb and 6 k subtypes of H C V and has a prospective