c语言flip用法,c-FLIPL 在白血病中的表达及临床意义

摘要：目的 探讨 c-FLIPL 在不同类型白血病中的表达水平及其临床意义。方法 应用实时 PCR 检测 103 例不同类型白血病患者骨髓单个核细胞 c-FLIPL mRNA 表达，包括急性淋巴细胞白血病(ALL)54 例(其中初诊 37 例、复发 5 例、完全缓解 12 例)、急 性髓细胞白血病(AML)38 例(其中初诊 24 例、复发 6 例、完全缓解 8 例)、初诊急性未分化白血病(AUL)2 例、初诊慢性粒细胞白 血病(CML)6 例、初诊慢性粒单核细胞白血病(CMML)3 例。应用流式细胞术对所选病例进行免疫表型检测。结果在初诊和 复发的白血病中 c-FLIPL mRNA 呈异常高表达趋势，二者间差异无统计学意义(P > 0.05)。初诊 AUL 及初诊 CML 的 c-FLIPL mRNA 表达高于其他初诊组，但差异无统计学意义(P > 0.05)。初诊 ALL、AML、AUL、CML、CMML 与对照组、完全缓解组比较， c-FLIPL mRNA 的差异均有统计学意义(P < 0.05)。 c-FLIPL mRNA 表达与患者危险度分级、初诊白细胞数 、血乳酸脱氢酶(LDH)、血羟丁酸脱氢酶(HBDH)、CD45、融合基因 TEL-AML1 相关，与患者年龄、性别、血纤维蛋白原及染色体异常无关。结论 白血病患者 c-FLIPL mRNA 表达异常增高，并与危险度分级、初诊白细胞数、血 LDH、血 HBDH 及预后密切相关。

Abstract:Objective To investigate the expression of c-FLIPL in leukemia and explore its clinical significance. Methods The expression level of c-FLIPL mRNA in bone marrow mononuclear cells was determined by real-time polymerase chain reaction(PCR)in 103 leukemia patients with different types of leukemia，including 54 cases of acute lymphocytic leukemia(ALL)with 37 newly diagnosed，5 relapsed，and 12 complete remission，38 cases of acute myeloid leukemia(AML)with 24 newly diagnosed，6 relapsed，and 8 complete remission，newly diagnosed 2 cases of acute undifferentiated leukemia(AUL)，6 cases of chronic myelocytic leukemia(CML)，and 3 cases of chronic myelomonocytic leukemia(CM- ML). The immunophenotype of patients were detected by flow cytometry. Results Expression level of c-FLIPL mRNA was higher in newly diagnosed and relapsed leukemia patients. There was no significant difference between newly diagnosed and relapsed leukemia patients(P > 0.05). Ex-pression level of c-FLIPL mRNA of AUL and CML was higher than that in other patients，but there was no significant difference(P > 0.05). Ex-pression level of c-FLIPL mRNA of all newly diagnosed and relapsed leukemia patients was significantly higher than that in control group and complete remission group(P < 0.05). The expression level of c-FLIPL mRNA was correlated with risk stratification，white blood cel(l WBC)，lactate dehydrogenase(LDH)，hydroxybutyrate dehydrogenase(HBDH)，CD45 and TEL-AML1，but was not associated with age，sex，fibrinogen and chromosome abnormality. Conclusion c - FLIPL mRNA is highly expressed in leukemia patients，and is closely related with risk stratification， WBC，LDH，HBDH and prognosis.

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R552

引用本文

迟昨非 ，何秋颖 ，杨威 ，傅煜 ，符爽 ，庄倩. c-FLIPL 在白血病中的表达及临床意义[J]. 中国医科大学学报, 2017, 46(2): 120-125.

CHI Zuofei，HE Qiuying，YANG Wei，FU Yu，FU Shuang，ZHUANG Qian. Expression of c-FLIPL in Leukemia and Its Clinical Significance[J]. Journal of China Medical University, 2017, 46(2): 120-125.