本文详细介绍了使用Tophat、Cufflinks和Cuffdiff进行RNA-seq数据分析的流程,包括转录组装、基因表达量化及差异表达分析。此外,还提到了利用featureCounts和DESeq2进行定量和差异分析的方法。

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Tophat –> Cufflink –> Cuffdiff

cufflinks 是做什么分析的

对每个样本进行转录组装

将所有样本进行转录组装

获得基因表达文件

cufflinks还有cuffdiff

根据文章设立的阈值,挑选出差异基因

 

 

 featureCounts 进行定量

采用DESeq2进行差异分析

 

应该是还有一个进行整合的文件脚本,以方便计算TPM值

 

 

 

 

 

 

 

 

#include "User_Task.h" #include "Drv_RcIn.h" #include "LX_FC_Fun.h" #include "ANO_DT_LX.h" void UserTask_OneKeyCmd(void) { ////////////////////////////////////////////////////////////////////// // ????? ////////////////////////////////////////////////////////////////////// static u8 one_key_takeoff_f = 1, one_key_land_f = 1, one_key_mission_f = 0; static u8 mission_step = 0; // ???? // ?? RC ?? if (rc_in.no_signal == 0) { // ??? RC ?? 6 ?????????? if (rc_in.rc_ch.st_data.ch_[ch_6_aux2] > 1700 && rc_in.rc_ch.st_data.ch_[ch_6_aux2] < 2200) { if (one_key_mission_f == 0) { one_key_mission_f = 1; // ???? mission_step = 1; // ?????? } } else { one_key_mission_f = 0; // ?????? } // ?????? if (one_key_mission_f == 1) { static u16 time_dly_cnt_ms = 0; // ????? switch (mission_step) { case 0: { // ?????,???? time_dly_cnt_ms = 0; mission_step += 1; // ??????? } break; case 1: { // ???? if (LX_Change_Mode(3)) { mission_step += 1; // ?????? } } break; case 2: { // ???? if (FC_Unlock()) { mission_step += 1; // ?????? } } break; case 3: { // ?? 2 ???????? if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; // ???? 20 ms } else { time_dly_cnt_ms = 0; mission_step += 1; // ??????? } } break; case 4: { // ??? 100 cm if (OneKey_Takeoff(150) == 1) { mission_step += 1; // ?????? } } break; case 5: { // ????? 4 ? if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; // ???? 20 ms } else { time_dly_cnt_ms = 0; // ????? mission_step += 1; // ?????? } } break; case 6: mission_step += Horizontal_Move(75,18,270); // Move horizontally break; case 7: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 8: { // ??? 0 cm if (Descend_to_Altitude(110,27) == 1) { mission_step += 1; // ?????? } } break; case 9: { // ?? 2 ??????? if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; // ???? 20 ms } else { time_dly_cnt_ms = 0; // ????? mission_step += 1; // ?????? } } break; case 10: mission_step += Horizontal_Move(50,18,270); // Move horizontally break; case 11: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 12: { // ??? 100 cm if (Ascend_to_Altitude(150,25) == 1) { mission_step += 1; // ?????? } } break; case 13: { // ????? 4 ? if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; // ???? 20 ms } else { time_dly_cnt_ms = 0; // ????? mission_step += 1; // ?????? } } break; case 14: mission_step += Horizontal_Move(50,18,270); // Move horizontally break; case 15: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 16: { // ??? 0 cm if (Descend_to_Altitude(110,27) == 1) { mission_step += 1; // ?????? } } break; case 17: { // ?? 2 ??????? if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; // ???? 20 ms } else { time_dly_cnt_ms = 0; // ????? mission_step += 1; // ?????? } } break; case 18: mission_step += Horizontal_Move(50,18,270); // Move horizontally break; case 19: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 20: mission_step += Horizontal_Move(90,23,0); // Move horizontally break; case 21: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 22: // ???????case???11 mission_step += Rotate_Left(180, 45); // ??180?,??30?/? break; case 23: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 24: mission_step += Horizontal_Move(50,18,270); // Move horizontally break; case 25: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 26: { // ??? 100 cm if (Ascend_to_Altitude(150,25) == 1) { mission_step += 1; // ?????? } } break; case 27: { // ????? 4 ? if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; // ???? 20 ms } else { time_dly_cnt_ms = 0; // ????? mission_step += 1; // ?????? } } break; case 28: mission_step += Horizontal_Move(50,18,270); // Move horizontally break; case 29: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 30: { // ?? 2 ??????? if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; // ???? 20 ms } else { time_dly_cnt_ms = 0; // ????? mission_step += 1; // ?????? } } break; case 31: { // ??? 0 cm if (Descend_to_Altitude(110,27) == 1) { mission_step += 1; // ?????? } } break; case 32: mission_step += Horizontal_Move(50,18,270); // Move horizontally break; case 33: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 34: { // ??? 100 cm if (Ascend_to_Altitude(150,25) == 1) { mission_step += 1; // ?????? } } break; case 35: { // ????? 4 ? if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; // ???? 20 ms } else { time_dly_cnt_ms = 0; // ????? mission_step += 1; // ?????? } } break; case 36: // ???????case???11 mission_step += Rotate_Left(225,45); // ??180?,??30?/? break; case 37: { // ????? 4 ? if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; // ???? 20 ms } else { time_dly_cnt_ms = 0; // ????? mission_step += 1; // ?????? } } break; case 38: mission_step += Horizontal_Move(100,25,0); // Move horizontally break; case 39: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 40: // ???????case???11 mission_step += Rotate_Right(45,25); // ??180?,??30?/? break; case 41: { // ????? 4 ? if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; // ???? 20 ms } else { time_dly_cnt_ms = 0; // ????? mission_step += 1; // ?????? } } break; case 42: { // ??? 0 cm if (Descend_to_Altitude(110,27) == 1) { mission_step += 1; // ?????? } } break; case 43: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 44: mission_step += Horizontal_Move(50,18,90); // Move horizontally break; case 45: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 46: { // ??? 100 cm if (Ascend_to_Altitude(150,25) == 1) { mission_step += 1; // ?????? } } break; case 47: { // ????? 4 ? if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; // ???? 20 ms } else { time_dly_cnt_ms = 0; // ????? mission_step += 1; // ?????? } } break; case 48: mission_step += Horizontal_Move(50,18,90); // Move horizontally break; case 49: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 50: { // ??? 0 cm if (Descend_to_Altitude(110,27) == 1) { mission_step += 1; // ?????? } } break; case 51: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 52: mission_step += Horizontal_Move(50,18,90); // Move horizontally break; case 53: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 54: mission_step += Horizontal_Move(50,18,0); // Move horizontally break; case 55: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 56: // ???????case???11 mission_step += Rotate_Left(180, 45); // ??180?,??30?/? break; case 57: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 58: mission_step += Horizontal_Move(50,18,90); // Move horizontally break; case 59: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 60: { // ??? 100 cm if (Ascend_to_Altitude(150,25) == 1) { mission_step += 1; // ?????? } } break; case 61: { // ????? 4 ? if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; // ???? 20 ms } else { time_dly_cnt_ms = 0; // ????? mission_step += 1; // ?????? } } break; case 62: mission_step += Horizontal_Move(50,18,90); // Move horizontally break; case 63: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 64: { // ??? 0 cm if (Descend_to_Altitude(110,27) == 1) { mission_step += 1; // ?????? } } break; case 65: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 66: mission_step += Horizontal_Move(50,18,90); // Move horizontally break; case 67: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 68: { // ??? 100 cm if (Ascend_to_Altitude(150,25) == 1) { mission_step += 1; // ?????? } } break; case 69: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 70: mission_step += Horizontal_Move(50,18,360); // Move horizontally break; case 71: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 72: mission_step += Horizontal_Move(50,18,90); // Move horizontally break; case 73: if (time_dly_cnt_ms < 2000) { time_dly_cnt_ms += 20; } else { time_dly_cnt_ms = 0; mission_step += 1; } break; case 74: { // ?? if (OneKey_Land() == 1) { mission_step = 0; // ????,???? } } break; default: break; } } } else { // ??????,?????? // ?????????? } }根据这段代码写pid控制
07-09
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