Deconvolution
All microscopes are limited by the laws of physics, and these laws state that when light passes through a medium, that light will bend. This is one of the most common causes of haze and blur in microscopy images. Deconvolution can correct this problem, not only removing the haze and blur, but restoring vital detail to datasets. This software can be used to sharpen fluorescence, brightfield, darkfield, confocal, ESM, DIC, and many other kinds of images.

Penicillum Chrysogenum stained with FUN1Viability kit; original and deconvolved images.

E. coli stained with Live/Dead kit; original and deconvolved images.

来源:http://web.utk.edu/~jbiggers/Deconvolution.html
GFP Tubulin in mouse fibroblasts, DAPI stained nuclei; original and deconvolved images.

本文介绍了使用反卷积技术纠正显微镜成像中由于光线折射造成的模糊问题。通过软件处理,不仅能去除模糊,还能恢复图像细节。适用于荧光、明场、暗场等多种类型的显微图像。
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