【评测】IDT CRISPR核酸内切酶解决方案

IDT 成立于 1989年，是基因组学领域开发的领先者，也是公认的定制核酸生产行业的领导者。IDT 凭借在 DNA 合成领域的领导能力，为基因组学应用开发了专有技术，例如下一代测序、CRISPR 基因组编辑、合成生物学、数字 PCR 和 RNA 干扰。通过 GMP 服务，IDT的产品被科学家用于研究多种癌症以及大多数遗传性和传染性疾病。IDT 在亚洲、欧洲和北美洲设有工厂，为一百多个国家及地区的超过十二万名生命科学界研究人员提供服务，每天生产的寡核苷酸数量 超过七万份。

 

 

 

技术优势

 

 

 

 

技术产品方案：

 

 

以上产品请微信搜索“泽平科技”公众号进入咨询。

 

参考文献：

• Kim KW, Tang NH, et al. (2018) A neuronal piRNA pathway inhibits axon regeneration in C. elegans. Neuron, 97 : 1–9.
• Tröder SE, Eber LK, et al. (2018) An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes.PLoS One, 13 (5) : e0196891.
• Brinkman EK, Kousholt AN, et al. (2018) Easy quantification of template-directed CRISPR/Cas9 editing. Nucleic Acids Res. doi: 10.1093/nar/gky164
• Gregg E. Homanics. (2018) Gene edited CRISPy critters for alcohol research. Alcohol. doi: 10.1016/j.alcohol.2018.03.001
• Andersson M, Turesson H, et al. (2018) Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery. Physiol Plant. doi: 10.1111/ppl.12731
• Ohtsuka M, Sato M, et al. (2018) i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases. Genome Biol, 19 : 25.
• Riddle MR, Aspiras AC, et al. (2018) Insulin resistance in cavefish as an adaptation to a nutrient-limited environment. Nature, 555 : 647–651.
• Seki A, Rutz S. (2018) Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells. J Exp Med. doi: 10.1084/jem.20171626
• Han X, Liu Z, et al. (2017) Cas9 ribonucleoprotein delivery via microfluidic cell-deformation chip for human T-Cell genome editing and immunotherapy . Adv Biosys, 1 : 1600007.
• Al Abdallah Q, Ge W, Fortwendel JR. (2017) A simple and universal system for gene manipulation in Aspergillus fumigatus: in vitro-assembled Cas9 guide RNA ribonucleoproteins coupled with microhomology repair templates. mSphere, 2 : e00446–17.
• di Pietro F, Valon L, et al.. (2017) An RNAi screen in a novel model of oriented divisions identifies the actin-capping protein Z β as an essential regulator of spindle orientation. Curr Biol, 27 : 2452–2464.
• Nachmanson D, Lian S, et al. (2017) CRISPR-DS: An efficient, low DNA input method for ultra-accurate sequencing. bioRxiv. doi: 10.1101/207027
• Schwinn MK, Machleidt T, et al. (2017) CRISPR-mediated tagging of endogenous proteins with a luminescent peptide. ACS Chem Biol.doi: 10.1021/acschembio.7b00549
• Xu MM, Pu Y, et al.. (2017) Dendritic cells but not macrophages sense tumor mitochondrial DNA for cross-priming through signal regulatory protein α signaling. Immunity, 47 : 363–37.
• Mikheikin A, Olsen A, et al. (2017) DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle. Nat Commun, 8 : 1665.
• Quadros RM, Miura H, et al. (2017) Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins . Genome Biology, 18 : 92.
• Wefers B, Bashir S, et al. (2017) Gene editing in mouse zygotes using the CRISPR/Cas9 system. Methods, 121–122 : 55–67.
• Rivera-Torres N, Banas K, et al.. (2017) Insertional mutagenesis by CRISPR/Cas9 ribonucleoprotein gene editing in cells targeted for point mutation repair directed by short single-stranded DNA oligonucleotides . PLoS One, 12 : e0169350.
• Agudelo D, Duringer A, et al.. (2017) Marker-free coselection for CRISPR-driven genome editing in human cells. Nature Methods, 14 :615–620.
• Luo L, Bokil N, et al.. (2017) SCIMP is a transmembrane non-TIR TLR adaptor that promotes proinflammatory cytokine production from macrophages . Nat Commun, 8 : 14133.
• Cuellar TL, Herzner AM, et al. (2017) Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia. J Cell Biol, 216 : 3535–3549.
• Jacobi AM, Rettig GR, Turk R, Collingwood MA, Zeiner SA, Quadros RM, Harms DW, Bonthuis PJ, Gregg C, Ohtsuka M, Gurumurthy CB, Behlke MA. (2017) Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes. Methods, 121–122 : 16–28.
• Kohler S, Wojcik M, et al.. (2017) Superresolution microscopy reveals the three-dimensional organization of meiotic chromosome axes in intact Caenorhabditis elegans tissue . Proc Natl Acad Sci USA, 114 : E4734–E4743.
• Grahl N, Demers EG, et al.. (2017) Use of RNA-protein complexes for genome editing in non-albicans Candida species . mSphere, 2 :e00218-17.